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| The C1si is equipped with two detectors—a traditional fluorescence one and one for spectral imaging—to manage a wide range of applications. |
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| One-shot Spectral Imaging • Effective for observing live cell dynamics. (Simultaneous 32-channel spectral-image acquisition) • Allows spectral imaging that is gentle on live cells because a wide wavelength spectrum can be captured with a single laser excitation. |
| True Spectral Imaging • Accurate fluorescence spectrum and true fluorescence colors can be obtained. • Wavelength resolution independent of confocal pinhole diameters,* a leak-free laser beam mechanism, sensitivity calibration between fluorescence detectors (using a NIST-compliant light source),* calibration of optical wavelength transmittance characteristics,* and wavelength calibration combine to achieve true spectral imaging. |
| Spectral Imaging Focusing on Brightness • Uncompromising optical design and signal processing perfectly trap fluorescence photons. • Newly developed Diffraction Efficiency Enhancement System (DEES) technology* and high-efficiency fluorescence transmission technology* are used in the spectral system. • Newly developed Dual Integration Signal Processing (DISP) technology* is used in the video signal-processing circuitry to achieve brighter spectral imaging. |
| Imaging of Unmixed Fluorescent Probes without Crosstalk • Effective in the observation of expression-multiplexed specimens stained with CFP, GFP, YFP, DsRed and similar reagents, as well as observation of localized protein molecules and FRET observation. Separation of autofluorescence is also possible. |
| * Patent pending |
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