Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, A1R+ is a powerful tool for the acquisition of intracellular dynamics and interaction.
Hybrid scanner for ultrafast photoactivation imaging
A1R+ has a hybrid scanner head that incorporates both an ultrahigh-speed resonant scanner and a high-resolution galvano scanner. Simultaneous photoactivation and ultrafast imaging using these two scanners allow acquisition of rapid changes after photoactivation and enables observation of intermolecular interaction.
What is a hybrid scanning head?
This mechanism allows flexible switching or simultaneous use of two scanners (resonant and galvano) with the use of a hyper selector.
Ultrafast imaging with a resonant scanner
A1'R+s resonant scanner has an ultrahigh resonance frequency of 7.8 kHz. It allows imaging of intercellular dynamics at 30 fps (512 x 512 pixels) and 420 fps (512 x 32 pixels), the world's fastest image acquisition. The field of view of the scanned area is approximately five times larger than that of the galvano scanner. The Nikon original optical clock generation method realizes high image quality even at the highest speed. The fiber-optic communication data transfer system can transfer data at a maximum of 4Gbps.
High-resolution imaging with a galvano scanner
The A1R+'s galvano scanner enables high-resolution imaging of up to 4096 x 4096 pixels. In addition, with the newly developed scanner driving and sampling systems, plus image correction technology, high-speed acquisition of 10 fps (512 x 512 pixels) is also possible.
Increased light detection efficiency realizes high image quality
The low-angle incidence method utilized on the dichroic mirrors increases fluorescence efficiency by 30%.
By employing the hexagonal pinhole, higher brightness equivalent to that of a circular pinhole is achieved.
Nikon's original dual integration signal processing technology (DISP) has been implemented in the image processing circuitry to improve electrical efficiency, resulting in an extremely high S/N ratio.
VAAS pinhole unit for bright, clear images
Nikon has developed an original confocal microscopy VAAS (Virtual Adaptable Aperture System, option) that can eliminate flare while retaining image brightness. Because of the deconvolution of the light that passes through the pinhole and the light that does not pass through the pinhole, acquisition of brighter images with less flare is possible. Different sectionings (slice thicknesses) can also be simulated after image acquisition.
Enhanced spectral imaging
High-speed spectral imaging
Acquisition of a 32-channel spectral image (512 x 512 pixels) with a single scan in 0.6 second is possible. Moreover, 512 x 32-pixel images can be captured at 24 fps.
Accurate and high-speed unmixing
Accurate spectral unmixing provides maximum performance in the separation of closely overlapping fluorescence spectra and the elimination of autofluorescence. Superior algorithms and high-speed data processing enable real time unmixing during image acquisition.
Actin of HeLa cell expressing H2B-YFP was stained with Phalloidin-Alexa488.
Spectral image in the 500-692 nm range captured with 488 nm laser excitation
Left: Spectral image, Right: Unmixed image (green: Alexa488, red: YFP)
Specimen courtesy of: Dr. Yoshihiro Yoneda and Dr. Takuya Saiwaki, Faculty of Medicine, Osaka University
Simultaneous excitation of four lasers
Three user-defined laser shields allow simultaneous use of four lasers selected from a maximum of nine colors, enabling broader band spectral imaging.
Filter-less intensity adjustment is possible by selecting desired spectral ranges from 32 channels that match the spectrum of the fluorescence probe in use and combining them to perform the filtering function.
Simple operation of complex applications
NIS—Elements C control software enables integrated control of the confocal imaging system, microscope and peripheral devices with a simple and intuitive interface. Diverse reliable analysis functions are also available.
System integration of Ti-E inverted microscope for multi-mode imaging is possible by equipping the confocal microscope system with N-SIM/N-STORM super resolution microscope system, TIRF system, spectral detector and Perfect Focus System. All systems can be controlled by a single NIS-Elements platform.