System Upgrades for Nikon's Inverted Microscope Ti

The Nikon Inverted Microscope Ti's expanded space "stratum structure" allows the use of multiple modules simultaneously without modifying the microscope body. This feature enables addition of an optional back port, allowing image capture with multiple cameras using two-tier dichroic fluorescence filter turrets.

System I

Simultaneous two-wavelength FRET imaging with multiple cameras

System II

Simultaneous acquisition of fluorescence and brightfield images

High Resolution Differential Interference Contrast Microscopy

Differential interference contrast microscopy transforms minute differences in refraction indexes of light passing through an unstained specimen, or optical path differences from the specimen surface shape, into a monochromatic shadow-cast images enabling observation.
Nikon uses short-shear prisms with VEC (Video Enhanced Contrast) to enable viewing and recording of extremely fine structures.