Fluorescence Filter Cubes

High-performance/high-quality fluorescence filter cubes for research that demands precise signal detection

Nikon offers a wide range of fluorescence filter cubes with high fluorescence acquisition efficiency to support imaging of a large variety of fluorophores and fluorescent proteins. The large product range includes longpass filter cubes for observation of single-stained samples and bandpass filter cubes suitable for detecting specific wavelengths from multiple-stained samples and unmixing.

  • Wavelengths with an extremely sharp rising edge characteristic.
  • High transmittance from 93% to 97% (both excitation and barrier filters).
  • High fluorescence acquisition efficiency, enabling reduced excitation intensity and sample fading.
  • The HQ series filters, made of glass with high dimensional accuracy, eliminate the possibility of image shifts when superimposing multi-color images and ensure accurate imaging even in the case of fluorophores with small Stokes shifts.

Longpass filter cubes

Filter Cube Excitation Spectral profile Filter diameter Excitation filter Dichroic mirror Barrier filter
UV-2A UV graph 25 mm 355/50 400 410
UV-1A graph 25 mm 365/10 400 390
V-2A V graph 25 mm 400/40 430 440
BV-2A BV graph 25 mm 420/40 455 460
B-2A B graph 25 mm 470/40 505 510
G-2A G graph 25 mm 535/50 575 580

UV-2A

UV-1A

V-2A

BV-2A

B-2A

G-2A

Bandpass filter cubes

Standard series

Filter Cube Spectral profile Filter diameter Excitation filter Dichroic mirror Barrier filter
DAPI graph 25 mm 375/28 415 460/60
GFP-B graph 25 mm 470/40 500 535/50
FITC graph 25 mm 480/30 505 535/45
TRITC graph 25 mm 540/25 565 605/55
Texas Red graph 25 mm 560/40 595 630/60

DAPI

GFP-B

FITC

TRITC

Texas Red

HQ series

Filter Cube Spectral profile Filter diameter Excitation filter Dichroic mirror Barrier filter
DAPI-U HQ graph 25 mm 395/25 425 460/50
CFP HQ graph 25 mm 436/20 455 480/40
GFP HQ graph 25 mm 470/40 495 525/50
FITC HQ graph 25 mm 480/40 510 535/50
YFP HQ graph 25 mm 500/20 515 535/30
Cy3 HQ graph 25 mm 535/40 565 590/40
mCherry HQ graph 25 mm 570/40 600 645/75
Cy5 HQ graph 25 mm 620/60 660 700/75

HQ series(for large FOV imaging with Ti2 microscope)

Filter Cube Spectral profile Filter diameter Excitation filter Dichroic mirror Barrier filter
DAPI-U HQ graph 32 mm 395/25 425 460/50
CFP HQ graph 32 mm 436/20 455 480/40
GFP HQ graph 32 mm 470/40 495 525/50
FITC HQ graph 32 mm 480/40 510 535/50
YFP HQ graph 32 mm 500/20 515 535/30
Cy3 HQ graph 32 mm 535/40 565 590/40
mCherry HQ graph 32 mm 570/40 600 645/75
Cy5 HQ graph 32 mm 620/60 660 700/75

DAPI-U HQ

CFP HQ

GFP HQ

FITC HQ

YFP HQ

Cy3 HQ

mCherry HQ

Cy5 HQ