Monochrome Microscope Camera

DS-Qi2

  1. Key Features
  2. Specifications/Dimensions

Capture low light fluorescence and large fields of view

High pixel density, high sensitivity and low noise are key features of the DS-Qi2 monochrome camera.

Large format CMOS sensors

Nikon manufactures CMOS sensors and imaging technologies for professional D-SLR cameras, which are now optimized for microscopy.

High sensitivity

Detects even faint fluorescent signals

7.3µm pixels, high quantum efficiency, and very low read noise allow the DS-Qi2 to even read faint fluorescent signals.

Indian Muntjac Deer Skin Fibroblast Cells, Cytoskeletal F-actin labeled with Alexa Fluor 488
Sample courtesy of: Michael Davidson and Florida State University

Excellent linearity

Reliable quantitative analysis made possible

With a linearity error of ±1%, the DS-Qi2 is a superb tool for measuring intensities in fluorescence samples, including time-based intensity measurement and ratiometric measurement.

High frame rate

Fast focusing, even with fluorescent images

Both 2.2 electrons read noise coupled with a large full-well capacity and 0.6 electrons dark current allow the acquisition of fluorescence images with very little noise.

Low noise

Acquires dim fluorescent signals with ultra-low noise

2.2 electrons read noise, and this, coupled with a large full-well capacity, allows the acquisition of fluorescence images with very little noise and a very high dynamic range.

LLC-PK1 cells expressing GFP-EB3 tubulin Low noise and large linear full well capacity allows the acquisition of large dynamic range in a single capture.
A captured image is shown intensity scaled to show both the brightest and dimmest areas.
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

Time-lapse photography

Fluorescent time-lapse imaging through integration with NIS-Elements software

With a large field of view and pixel density, and low noise, the DS-Qi2 is ideal for time-resolved imaging applications.

Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the timelapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path.
DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped timelapse sequence from a large FOV image.
Objective: CFI Plan Apochromat Lambda 60X Oil (NA: 1.4)
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

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