High-sensitivity spectral image acquisition
With a GaAsP PMT, the A1-DUVB-2 tunable emission detector delivers flexible detection of fluorescent signals with higher sensitivity, in both the galvano and resonant imaging modalities.
Variable acquisition wavelength range
User-defined emission bands can collect images within a selected wavelength range, replacing the need for fixed bandwidth emission filters.
Users can define the emission bandwidth range to as little as 10nm. Spectral images of multi-labeled specimens can be acquired by capturing a series of spectral images while changing detection wavelengths.
Based on the application, virtual bandpass mode and continuous bandpass modalities are selectable on the A1-DUVB-2.
HeLa cells labeled with five-color fluorescence, Nucleus: DAPI, Vimentin: Alexa Fluor® 488, Lamin: Alexa Fluor® 568, Tubulin: Alexa Fluor® 594, Actin: Alexa Fluor® 633
Specimen courtesy of: Dr. Tadashi Karashima, Department of Dermatology, Kurume University School of Medicine
Optional second channel detector
An optional second GaAsP PMT provides flexibility in detection. Users can divert selected wavelengths to the second fixed bandwidth emission channel by inserting a dichroic mirror, while simultaneously utilizing the user-definable emission band on the first channel. The second detector allows FRET, ratio imaging and other applications requiring simultaneous multi-channel imaging.
Accurate spectral unmixing
Multi-channel images acquired with the A1-DUVB-2 can be spectrally unmixed by using the spectra of reference samples, or the spectra within the acquired images.