Confocal Microscope

A1+ / A1R+

Highest performance optics for confocal imaging

A selection of high numerical aperture (NA) objectives are available, which provide chromatic aberration correction for UV to near-infrared.

CFI SR HP Plan Apochromat Lambda S 100XC Sil

By using silicone oil that has a refractive index closely matching that of live cells as its immersion liquid, this lens allows high resolution imaging of thick samples and is suitable for long-term time-lapse imaging.

  • Numerical aperture: 1.35
  • Chromatic aberration correction: from visible to UV
  • Nano Crystal Coat applied.

CFI75 Apochromat 25XC W

This lens is suitable for multicolor imaging of living cells.

  • Working distance: 2.0 mm
  • Numerical aperture: 1.10
  • Chromatic aberration correction: from visible to near-IR
  • Nano Crystal Coat applied.

CFI Apochromat Lambda S 40XC WI

Its high NA for water immersion objectives provides brighter and higher-resolution images and makes this lens ideal for confocal live cell imaging.

  • Numerical aperture: 1.25
  • Chromatic aberration correction: from UV through to near IR
  • Nano Crystal Coat applied.

CFI Plan Apochromat VC 60XC WI

This lens’ chromatic aberration correction up to the UV range enables accurate multicolor confocal imaging

  • Chromatic aberration correction: the full visible wavelength range over 405 nm
  • Superior image flatness

CFI Apochromat TIRF 60XC Oil

This lens has the industry’s highest NA, providing unparalleled resolution and efficient acquisition of fluorescent signals in confocal imaging.

  • Numerical aperture: 1.49

Nano Crystal Coat for superior transmission

Nikon’s exclusive Nano Crystal Coat is an anti-reflective coating consisting of ultra-fine crystalline particles. This forms a coarse structure that enables lower refractive indices, facilitating the passage of light through the lens rather than reflecting it, thus providing superior light transmission.

Objectives for confocal microscopy
CFI SR HP Plan Apochromat Lambda S 100XC Sil NA 1.35 WD 0.30 Nano Crystal Coat
CFI Plan Apochromat Lambda 10X NA 0.45 WD 4.00 Nano Crystal Coat
CFI Plan Apochromat Lambda 40XC NA 0.95 WD 0.21 Nano Crystal Coat
CFI Plan Apochromat VC 20X NA 0.75 WD 1.00
CFI Plan Apochromat VC 60X WI NA 1.20 WD 0.31-0.28
CFI Plan Apochromat IR 60XC WI NA 1.27 WD 0.18-0.16 Nano Crystal Coat
CFI Apochromat Lambda S 40XC WI NA 1.25 WD 0.20-0.16 Nano Crystal Coat
CFI Apochromat LWD Lambda S 40XC WI NA 1.15 WD 0.62-0.58 Nano Crystal Coat
CFI Apochromat Lambda S 60X Oil NA 1.40 WD 0.14 Nano Crystal Coat
CFI Apochromat TIRF 60XC Oil NA 1.49 WD 0.14-0.08 (23℃)
WD 0.16-0.10 (37℃)
CFI Apochromat TIRF 100XC Oil NA 1.49 WD 0.14-0.08 (23℃)
WD 0.15-0.09 (37℃)
CFI75 Apochromat 25XC W NA 1.10 WD 2.00 Nano Crystal Coat

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