Multiphoton Confocal Microscope

A1 MP+ / A1R MP+

  1. Key Features
  2. Objectives
  3. Sample Images
  4. Specifications/Dimensions

Highest performance optics for multiphoton confocal imaging

A selection of high numerical aperture (NA) objectives are available, which provide chromatic aberration correction up to the near-infrared range, and allow multiphoton imaging with highly efficient excitation.

CFI90 20XC Glyc [NEW]

This objective includes a correction collar that accommodates refractive indices ranging from 1.44 to 1.50 and is compatible with a variety of immersion media and tissue-clearing agents. With its high NA, large field-of-view, and ultra-long working distance, this objective enables observation of large samples and whole organs with exceptional clarity and throughput. It also provides superior chromatic aberration correction and transmittance in the NIR wavelength range for multiphoton applications.

  • Working distance: 8.2 mm
  • Numerical aperture: 1.00
  • Chromatic aberration correction: from 588nm to 1300nm
  • Nano Crystal Coat applied.

CFI Plan Apochromat 10XC Glyc

Correction for refractive indices from 1.33 to 1.51 enables deep 3D-imaging of tissues cleared with a variety of optical clearing agents.

  • Working distance: 5.50 mm
  • Chromatic aberration correction: from UV through to near IR
  • Nano Crystal Coat applied.

CFI75 Apochromat 25XC W 1300

This lens is perfect for deep multiphoton imaging, achieving both a high NA and long working distance, as well as correcting spherical aberrations caused by sample thickness. Its chromatic aberration correction in IR range is powerful for simultaneous two-wavelength excitation imaging.

  • Working distance: 2.0 mm
  • Numerical aperture: 1.10
  • Chromatic aberration correction: from visible to 1300nm
  • Nano Crystal Coat applied.

CFI Apochromat Lambda S 40XC WI

Its high NA for water immersion objectives provides brighter and higher resolution images and is ideal for confocal live cell imaging.

  • Numerical aperture: 1.25
  • Chromatic aberration correction: from UV through to near IR
  • Nano Crystal Coat applied.

CFI Plan Apochromat VC 60XC WI

Its chromatic aberration correction up to UV range enables accurate multicolor confocal imaging.

  • Chromatic aberration correction: the full visible wavelength range over 405 nm
  • Superior image flatness

CFI Apochromat TIRF 60XC Oil

Its industry’s highest NA provides unparalleled resolution and efficient acquisition of fluorescent signals in confocal imaging.

  • Numerical aperture: 1.49
  • Chromatic aberration correction: from UV through to near IR

Nano Crystal Coat for superior transmission

Nikon’s exclusive Nano Crystal Coat is an anti-reflective coating consisting of ultra-fine crystalline particles. This forms a coarse structure that enables lower refractive indices, facilitating the passage of light through the lens rather than reflecting it, thus providing superior light transmission.

Objectives for multiphoton microscopy
CFI90 20XC Glyc* NA 1.00 WD 8.20 Nano Crystal Coat
CFI Plan Apochromat 10XC Glyc NA 0.50 WD 5.50 Nano Crystal Coat
CFI75 Apochromat 25XC W 1300 NA 1.10 WD 2.00 Nano Crystal Coat
CFI75 Apochromat 25XC W NA 1.10 WD 2.00 Nano Crystal Coat
CFI75 LWD 16X W NA 0.80 WD 3.00
CFI Apochromat NIR 40X W NA 0.80 WD 3.50
CFI Apochromat LWD Lambda S 40XC WI NA 1.15 WD 0.62-0.58 Nano Crystal Coat
CFI Apochromat LWD Lambda S 20XC WI NA 0.95 WD 0.95 Nano Crystal Coat
CFI Apochromat Lambda S 40XC WI NA 1.25 WD 0.20-0.16 Nano Crystal Coat
CFI Plan Fluor 20XC MI NA 0.75 WD 0.51-0.35 (Oil)
0.51-0.34 (Water)
0.49-0.33 (glycerin)
CFI Plan Apochromat IR 60XC WI NA 1.27 WD 0.18-0.16 Nano Crystal Coat
CFI Plan Apochromat VC 60X WI NA 1.20 WD 0.31-0.28
CFI Apochromat TIRF 60XC Oil NA 1.49 WD 0.14-0.08 (23℃)
WD 0.16-0.10 (37℃)
  • *Compatible with Ni-E/FN1 microscopes with a dedicated nosepiece

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