Multiphoton Confocal Microscope

A1 MP+ / A1R MP+

  1. Key Features
  2. Objectives
  3. Sample Images
  4. Specifications/Dimensions
A1 MP+ A1R MP+
Scan head input/output port 3 laser input ports, 3 signal output ports for standard, spectral and optional detector*1
Laser for multiphoton microscopy Compatible laser Mai Tai HP/eHP DeepSee, InSight DS+*2, InSight DS+ Dual Option*3, InSight X3*2, InSight X3 Dual Option*3 (Spectra-Physics), Chameleon Vision II, Chameleon Discovery*3 (Coherent)
Modulation Method: AOM (Acousto-Optic Modulator) device
Control: power control, return mask, ROI exposure control
Incident optics 700 - 1080 nm*4,*5, 700 - 1300 nm*2,*3, auto alignment
Laser for confocal microscopy (option) LU-N3 3 laser unit 405 nm, 488 nm, 561nm lasers are installed; built-in AOTF (Cannot used with A1-DUS spectral detector)
LU-N4/LU-N4S 4laser unit 405 nm, 488 nm, 561 nm, 640 nm lasers are installed; built-in AOTF (LU-N4 cannot be used with A1-DUS spectral detector)
LU-NV Laser unit Compatible laser: 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm, 647 nm; built-in AOTF
NDD for multiphoton microscopy Type Compatible with 1080 nm: Episcopic GaAsP NDD (for Ti2-E/Ni-E/FN1), Diascopic GaAsP NDD (for Ni-E/FN1); Detectable wavelength range 380 - 650 nm*6
Compatible with 1300 nm: Episcopic GaAsP NDD (for Ti2-E/Ni-E/FN1); Detectable wavelength range 380 - 750 nm
Detector 4 PMTs (3 GaAsP PMTs and 1 Multi-Alkali PMT)
Filter cube 450/50, 492, 525/50, 575/25, 610/75, 629/53
Standard fluorescence detector (option) Detectable wavelength range 400 - 750 nm (400 - 650 nm when using IR laser)
Detector A1-DU4-2 4 Detector Unit: 4 Multi-Alkali PMTs
A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs
Filter cube 6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths for multiphoton/confocal observation: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 594LP, 595/50, 700/75
Diascopic detector (option) Detectable wavelength range 440 - 645 nm
Detector Multi-Alkali PMT
FOV Square inscribed in a Φ18 mm circle
Image bit depth 4096 gray intensity levels (12 bit)
Scan head Type A1-SHSM-2 A1-SHRM-C
Standard image acquisition Scanner: galvano scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed:
Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction)
Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction) *7
Zoom: 1-1000x continuously variable
Scan mode: X-Y, X-T, X-Z, XY rotation, Free line, Line-Z
High-speed image acquisition Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz),
galvano scanner (Y-axis)
Pixel size: max. 1024 x 1024 pixels
Scanning speed: 15 fps (1024 x 1024 pixels), 30 fps (512 x 512 pixels), 60 fps (256 x 256 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed)
Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Scan mode: X-Y, X-T, X-Z
Acquisition method: High-speed image acquisition, Simultaneous photoactivation and image acquisition
IR laser wavelength range 700 - 1080 nm*4,*5, 700 - 1300 nm*2,*3
Dichroic mirror Low-angle incidence method Position: 8
Standard filter: 405/488, 405/488/561, 400-457/514/IR, 405/488/543/638, IR total reflection*2,*3, BS20/80, IR
Simultaneous stimulation option
Optional hyper selector
  • Visible Stimulation/Visible imaging: Stimulation by 405nm, Imaging by 488-750nm
  • Visible stimulation/IR imaging: Stimulation by 405nm, 488nm or 561nm (selectable by NDD dichroic mirror), Imaging by 800nm-1080nm (1080nm configuration), 820nm-1080nm (1300nm configuration)
Optional 1st dichroic mirror :High reflection mirror 405,488,561,800-1080
Pinhole 12 - 256 μm variable (1st image plane)
Spectral detector (option) A1-DUS spectral detector unit Number of channels: 32
Wavelength detection range: 400 - 750 nm
Spectral image acquisition speed: 4 fps (256 x 256 pixels)
Maximum pixel size: 2048 x 2048 (Spectral mode/Virtual filter mode)
Wavelength resolution: 2.5/6.0/10.0 nm, wavelength range variable in 0.25 nm steps
Compatible with galvano scanner only
A1-DUVB-2 GaAsP detector unit Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (A1-DUVB-OP) with a user-defined dichroic mirror and barrier filter
Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest:320 nm
Maximum pixel size: 4096 x 4096 (CB mode/VB mode)
Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps
Compatible with galvano and resonant scanners
Compatible microscopes ECLIPSE Ti2-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type)
Z step Ti2-E: 0.02 µm, FN1 stepping motor: 0.05 µm, Ni-E: 0.025 µm
Option Motorized XY stage (for Ti2-E/Ni-E), High-speed Z stage (for Ti2-E), High-speed piezo objective-positioning system (for FN1/Ni-E)
Software Display/image generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Image format JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS
Application FRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control computer OS Windows 10 Pro 64bit, English version or Japanese version OS Version 1704
Windows 7 Professional, 64bit, SP1 English version or Japanese version Windows Update KB3118401 or later
CPU Intel Xeon E5-2643v4 (3.40GHz, 6 cores, 20MB, 2400MHz) or higher
RAM 16GB, 32GB or 64GB
HDD 1st HP Z Turbo G2 512GB PCIe M.2 SSD, 2nd SATA 2TB
Optical Drive Super Multi drive, up to x 16 speed or higher
Graphics NVIDIA Quadro K620/ K2200/ K4200/ M2000/ M4000/ M5000 (PCI Express / two-screen split display supported)
Extension slot Two PCI Express 3.0 (x16) slots (one slot to be used for graphics), One PCI Express 3.0 (x8 mechanical, x4 electrical) slot, One PCI Express 2.0 (x8 mechanical, x4 electrical) slot, One PCI Express 2.0 (x1) slot
LAN port 10/100/1000 Network/Interfacex2 (for connection to controller, for connection to external LAN)
Monitor 1600 x 1200 or higher resolution, dual monitor configuration recommended
Vibration isolated table 1500 (W) x 1500 (D) mm required (for FN1/Ni-E), or 1800 (W) x 1500 (D) mm required (for Ti2-E)
  • *1FCS/FCCS/FLIM is possible in combination with third-party systems.
  • *2When using accessories with 1300 nm.
  • *3When using accessories with 1300 nm and Dual IR.
  • *4When using accessories with 1080 nm.
  • *5When using accessories with 1080 nm and visible light photoactivation/IR imaging.
  • *6400 - 650 nm when using diascopic NDD.
  • *7Fast mode is compatible with zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line, CROP, ROI, Spectral imaging, Stimulation and FLIM.

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