Imaging Software

NIS-Elements

  1. Overview
  2. Key Features
  3. Options
  4. NIS-Elements HC
  5. Table of features

Image Acquisition

NIS-Elements offers the most suitable image acquisition for various applications with the integrated control of the camera, motorized microscope and peripheral devices.

Multichannel (multi color)Advanced ResearchBasic Research

NIS-Elements can acquire full bit depth multi-color images, combining multiple fluorescence wavelengths and different illumination methods (DIC, phase contrast etc.), while offering independently scalable channels

Z-seriesAdvanced ResearchBasic ResearchDocumentation

Through motorized focus control, NIS-Elements reconstructs and renders 3D images from multiple Z-axis planes.

Time LapseAdvanced ResearchBasic ResearchDocumentation

Time lapse imaging in NIS-Elements is easily configurable simply by setting the time interval and duration of capture.
The Perfect Focus System of the motorized inverted microscope Ti2-E enables high-accuracy image capture without focus drifting even during extended time time-lapse experiments.

Multipoint ExperimentsAdvanced ResearchBasic ResearchDocumentation

NIS-Elements’ motorized stage control offers automated travel to multiple stage points of the sample of a multi-well plate or dish. Stage points are memorized and can be saved and loaded for future imaging sessions.

Image Stitching (Large image)Advanced ResearchBasic ResearchDocumentation

Large Image Acquisition generates a single high-magnification wide field-of-view image by automatically stitching multiple adjacent frames from a multipoint acquisition using a motorized stage or from multiple single images captured from a previous session.
Users can easily select image acquisition ranges and areas from low magnification images.

Scan Large Images with Macro Image

When using NIS-Elements with the Ni-E microscope and the DS-L4, the image-acquisition area can be easily specified with the image-capture button on the Ni-E while the image is being observed through the Ni-E’s eyepieces or viewed as a low-magnification image on the DS-L4 screen.

Optical ConfigurationAdvanced ResearchBasic ResearchDocumentation

Presets or ‘Optical Configurations’ can be saved for each observation method such as FITC fluorescence and DIC imaging, memorizing the settings of the microscope, camera and peripheral devices. The optical configurations are created through a one click set up and are displayed as icons in the tool bar for easy access and use.

Display and Data Processing

Various methods are available for displaying and processing captured images and datasets.

Multi-dimensional Image DisplayAdvanced ResearchBasic Research

NIS-Elements displays time lapse, multi-channel, multiple X, Y, Z positions in an intuitive layout, which allows for automatic playback and the ability to select subsections of the data to be saved as a new file.

Merge ChannelsAdvanced ResearchBasic ResearchDocumentation

Multiple single channel images (ex., two from three-channel acquisition images) can be merged together to create an overlay of full depth separately scalable images. With AR and BR, images can be merged by simply dragging the tab of one image onto another image. With D, images are merged by selecting each image for red, blue, green and brightfield channels.

Image Processing

Image Filtering, Color AdjustmentAdvanced ResearchBasic ResearchDocumentation

  • *Usable functions vary depending on the package.

With NIS-Elements image processing tools, it is possible to modify image display and feature extraction using various filters for, for example, sharpness, smoothing and detection. White balance and RGB/HIS balance adjustment are additional available options.

Arithmetic operation (Image arithmetic)Advanced ResearchBasic ResearchOptionDocumentation

NIS-Elements enables arithmetic operations such as addition, subtraction, multiplication and division on an image or between multiple images. Arithmetic operation between multiple images is also possible.

Arithmetic operation (Image averaging)Advanced Research

NIS-Elements reduces the noise of an image by averaging multiple sequential images such as time-lapse images. Rolling averaging that does not reduce frame rate is available as well.

Z-Series Image DisplayAdvanced ResearchBasic ResearchDocumentation

  • *Volume view and slice view are only possible with AR and BR.

Z-series images can be displayed in various formats such as max. and min. projections, X-Z axis and Y-Z axis cross-sectional slice view and 3D volume view. Rotatable 3D volume rendered views from 3D datasets are easily converted to an AVI or MOV format for file sharing and export.

Measurement and Analysis

Manual Measurement (Interactive Measurement) and Image AnnotationAdvanced ResearchBasic ResearchDocumentation

Interactive Measurement allows easy measurement of length and area by drawing lines or an object directly on the image. The results can be attached to the image, and also exported as text or to an Excel spreadsheet. Annotations such as arrows, circles, squares, text are also available display options.

Histogram/Intensity Line Profile/Intensity Surface PlotAdvanced ResearchBasic ResearchDocumentation

Histogram measurement measures the intensity distribution of pixels across the whole image or a defined region. An intensity line profile measurement shows the intensity distribution on a defined line. The Intensity Surface plot shows the intensity distribution of an image with the height of the z-axis line.

ROI StatisticsAdvanced ResearchBasic Research

  • *Usable functions vary depending on the package.

Common pixel measurements such as area, maximum or minimum intensity are possible with the user defined ROI (Region Of Interest). ROI or multiple ROIs statistic results for a single image or a multi-dimensional dataset are displayed and easily exported as text or an excel file.

Auto Measurement (Object Counting)Advanced ResearchBasic ResearchOptionDocumentation

Auto measurement measures the number or area of objects which are extracted from images by the creation of a binary layer through thresholding using RGB/HIS or intensity values. The results can be listed or exported as text or an excel file. It is possible to save and reuse thresholding parameters.

Pixel ClassifierAdvanced ResearchBasic ResearchOptionDocumentation

This function classifies each pixel in the image with RGB/HIS and intensity across the whole image. Results are reported in percentage and it possible to save and reuse parameters across a large sample of images. Multiple binary layers are also displayed with multiple colors on the image and are available with other analysis tools within the software package.

Time (Intensity) MeasurementAdvanced ResearchOptionBasic Research

Time measurement creates a graph of sequential intensity changes while time-lapse imaging or from captured time-lapse images. Ratio view function* allows the measurement of the ratio of two wavelengths across multiple ROIs and shows the ratio value by pixel. Numeric data and graph images are exportable and the measurements on the graph are available as well. (* Only with AR)

GUI Option

Industrial Simple GUIDocumentation

With D package, the simple GUI mode provides controls for the most common operations such as image capture and simple measurement.

[fig.]
Standard GUI mode: Displays all functions of D package
[fig.]
Simple GUI mode: Display only image capturing and measurement

Dark Color SchemeAdvanced ResearchBasic Research

This popular display option mode has a brightness level interface color palette suitable for use in a dark microscopy room.

Layout ManagerAdvanced ResearchBasic ResearchDocumentation

Layout manager enables customizing layouts of controls, toolbars and menus and application (image acquisition or measurement). Saving custom layouts is possible and accessible through one-click tab access.

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