Long-term, time-lapse imaging without focus drift
Hematopoietic differentiation of hES cells
Time-lapse movie showing morphological changes from day 0 to day 28 (hematopoietic differentiation of hES cells). The images were taken in combination with perfusion components at 10x magnification every 8 minutes for 4 weeks.
Courtesy of Dr. Akira Niwa, Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University
Long-term time-lapse observation of rat hippocampal neurons
Membrane-anchored GFP of rat hippocampal neurons was captured in 120-hour time-lapse imaging.
Courtesy of Dr. Chieko Nakada, Dr. Yuuri Nemoto, Dr. Hiroko Hijikata, Dr. Akihiro Kusumi, Institute for Integrated Cell-Material Sciences, Kyoto University
Time-lapse observation of fertilized mouse embryo up to the blastocyst stage
Time-lapse observation of fertilized mouse embryo up to the blastocyst stage for a total 105 hours. During observation, medium is replaced with blastocyst medium.
40x (before medium replacement)
80x (after medium replacement)
Courtesy of Ronny Janssens, Centrum voor Reproductieve Geneeskunde, Laboratorium IVF, Z-VUB Brussels
Scratch assays of MDCK cells
Scratch assays of MDCK cells, at 20x magnification every one minute for 3 hours.
Courtesy of Laurence Mery, INSERM, Universite Paris-Sud Orsay, France (Journal of Cell Science 123, 1449-1459)
Colonization of mouse iPS cells
Colonization of mouse iPS cells initiated with Oct4, Sox2, cMyc, Klf4 genes, at 20x magnification every 10 minutes for 60 hours on the MEF feeder.
Courtesy of System BioScience Inc.