Super Resolution Microscope

N-SIM

  1. Key Features
  2. Specifications/Dimensions
  3. Principle
  4. Sample Images
Lateral resolution (FWHM of beads in xy) 115 nm*1 in 3D-SIM mode, 86 nm*2 in TIRF-SIM mode
Axial resolution (FWHM of beads in z) 269 nm*1 in 3D-SIM mode
Image acquisition time Up to 0.6 sec/frame (TIRF-SIM/2D-SIM)
Up to 1 sec/frame (3D-SIM)
Reconstructed image size 1024 x 1024 pixels, 2048 x 2048 pixels*3
Imaging mode TIRF-SIM
2D-SIM
3D-SIM (Reconstruction method: slice, stack)
Multi-color imaging Up to 5 colors
Simultaneous
multi-color imaging
Two colors
Compatible laser LU-NV series laser unit
Standard: 405 nm, 488 nm, 561 nm, 640 nm
Option: 445 nm, 514 nm
Laser combination: 405 nm/445 nm/488 nm/561 nm/647 nm
Compatible microscope Motorized inverted microscope ECLIPSE Ti2-E
  • Perfect Focus System
  • Motorized XY stage with encoders
  • Piezo Z stage
Objective CFI SR HP Plan Apochromat Lambda S 100XC Sil (NA1.35)
CFI SR HP Apochromat TIRF 100XC Oil (NA 1.49)
CFI SR Apochromat TIRF 100XAC Oil (NA 1.49)
CFI SR Plan Apochromat IR 60XC WI (NA 1.27)
CFI SR Plan Apochromat IR 60XAC WI (NA 1.27)
CFI Plan Apochromat Lambda 60XC (NA 0.95)*4
CFI Plan Apochromat Lambda 40XC (NA 0.95) *4
Camera iXon Ultra DU-897U EMCCD camera (Andor Technology Ltd.)
ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.)
Software NIS-Elements Ar
NIS-Elements C (for Confocal Microscope A1+/A1R+)
Both require additional software modules NIS-A 6D and N-SIM Analysis
Operating conditions 20 ℃ to 28 ℃ (± 0.5 ℃)
  • *1These values are measured using 100 nm diameter beads excited with 488 nm laser. Actual resolution is dependent on laser wavelength and optical configuration.
  • *2This value is measured using 40 nm diameter beads excited with 488 nm laser. Actual resolution is dependent on laser wavelength and optical configuration.
  • *3With ORCA-Flash 4.0 sCMOS camera
  • *4Supports 2D-SIM and 3D-SIM (slice reconstruction).

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