Super Resolution Microscope

N-SIM E

  1. Key Features
  2. Specifications/Dimensions
  3. Principle
Lateral resolution (FWHM of beads in xy) 115 nm* in 3D-SIM mode
Axial resolution (FWHM of beads in z) 269 nm* in 3D-SIM mode
Image acquisition time Up to 1 sec/frame (3D-SIM)
Reconstructed image size 1024 x 1024 pixels, 2048 x 2048 pixels**
Imaging mode 3D-SIM
Reconstruction method: slice, stack (option)
Multi-color imaging Up to 3 colors
Simultaneous
multi-color imaging
Compatible Laser LU-N3-SIM laser unit
488 nm, 561 nm, 640 nm
Compatible microscope Motorized inverted microscope ECLIPSE Ti2-E
  • Perfect Focus System
  • Motorized XY stage with encoders
  • Motorized barrier filter wheel
  • Piezo Z stage (option)
Objective CFI SR HP Plan Apochromat Lambda S 100XC Sil (NA1.35)
CFI SR HP Apochromat TIRF 100XC Oil (NA 1.49)
CFI SR Apochromat TIRF 100XAC Oil (NA 1.49)
CFI SR Plan Apochromat IR 60XC WI (NA 1.27)
CFI SR Plan Apochromat IR 60XAC WI (NA 1.27)
CFI Plan Apochromat Lambda 60XC (NA 0.95)***
CFI Plan Apochromat Lambda 40XC (NA 0.95)***
Camera ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.)
Software NIS-Elements Ar
NIS-Elements C (for Confocal Microscope)
Both require additional software modules NIS-A 6D and N-SIM Analysis
Operating conditions 20 ℃ to 28 ℃ (± 0.5 ℃)
  • *These values are measured using 100 nm diameter beads excited with 488 nm laser. Actual resolution is dependent on laser wavelength and optical configuration.
  • **With ORCA-Flash 4.0 sCMOS camera
  • ***Supports slice reconstruction.

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