Attend ISSCR

ANNUAL METTING ISSCR 2016EXHIBITOR

The ISSCR's annual meeting has become the world's premier stem cell research event. The meeting serves as the largest forum for stem cell and regenerative medicine professionals from around the world. During the exhibition, Nikon will present its advance imaging products and applications. There will also be an Innovation Showcase that will highlight Nikon's Live-cell based drug-screening method for Neuronal degeneration.

EXIBITION BOOTH

VENUE: West Hall Level 1, Moscone Center,

San Francisco, CA, USA

BOOTH: 1318

Innovation showcase provided by Nikon

Developing a live cell system for visualizing neuronal degeneration

Professor Lee L. Rubin, PhD

  • SPEAKER:
    Professor Lee L. Rubin, PhD

    Director of HSCI's Therapeutic Screening Center Professor in Harvard University, USA

  • DATE: Thursday, June 23, 2016
  • TIME: 11:30 a.m. - 12:30 p.m
  • VENUE: Level 2, Rooms 2009/2011
  • E-mail: Support.Biostation@nikon.com

Applications

Nikon BioStation CT allows for long term time-lapse imaging of cells using phase contrast and fluorescence.

Imaging of the direct induction from mouse fibroblasts to Neural stem cells and neurons

Stem Cells. 2012 Jun;30(6) : 1109-19

  • Courtesy
    Professor Hideyuki Okano and Dr. Takeshi Matsui Department of Physiology, Keio University School of Medicine
  • Magnification : 4X
  • Culture period : 18 days
  • Imaging interval : 4 hours

The long term images from the BioStation CT are analyzed using advanced detection and tracking software

Neural differentiation analysis

iPS colony tracking - Dr. Miho K Furue and Dr. Kinehara

NK 92 cell cytotoxicity for immunotherapy - CCRM

Dendrite detection - Prof. James Ellis (Hospital for Sick Children-Toronto) and CCRM

Features

Nikon BioStation CT is an advanced, automated cell culture observation instrument specialized for stem cell research.
Ideally suited for live cell imaging, Nikon's BioStation CT maintains a stable environment during microscopic image acquisition.

Live Cell Analysis

Whole well scan imaging enables clear detection of iPS cell colonies or differentiated cells, which are difficult to detect because of its low induction efficiency. Advanced image analysis technology facilitates live cell screening, quantitative evaluation and a better understanding of the reprogramming/differentiation process and disease modeling. Imaging and analysis become processed automatically by separate PC with no stress. The left Figure shows the result of automated classification into iPS and non-iPS cell colonies from the phase contrast images. (The entire area of a 100mm dish)

Multi Sample

The BioStation CT can incubate up to 30 vessels at one time. Researchers can examine different culture conditions at one time.

Vessel Type

User can use various culture vessels (Well-plate, dish, flask)

Stable Cultivation

Temperature, Humidity, CO2 concentration and O2 concentration are controlled by accurate sensor and feedback system.

User's List

  • Harvard Stem cell Institute
  • Morgridge Institute
  • Diabetes Center at UCSF
  • UCR Stem Cell Core Facility
  • USC Stem Cell Core
  • Newcastle University
  • University Hospital Bern
  • Gurdon Institute
  • Masaryk University
  • Wellcome Trust Centre for Stem Cell Research
  • Centre for Stem Cell Biology University of Sheffield
  • China Agricultural University
  • Kyoto University
  • Keio University
  • National Institute of Biomedical
  • Innovation
  • Riken
  • Tokyo Women's Medical University

Articles

Neural Stem Cells Directly Differentiated from Partially Reprogrammed Fibroblasts Rapidly Acquire Gliogenic Competency

Takeshi Matsui, Morito Takano, Kenji Yoshida, Soichiro Ono, Chikako Fujisaki, Yumi Matsuzaki, Yoshiaki Toyama, Masaya Nakamura, Hideyuki Okano, Wado Akamatsu
Stem Cells. 2012 Jun;30(6):1109-19

Protein Kinase C Regulates Human Pluripotent Stem Cell Self-Renewal

Masaki Kinehara, Suguru Kawamura, Daiki Tateyama, Mika Suga, Hiroko Matsumura, Sumiyo Mimura, Noriko Hirayama, Mitsuhi Hirata, Kozue Uchio-Yamada, Arihiro Kohara, Kana Yanagihara, Miho K. Furue
PLOS ONE. January 2013 | Volume 8 | Issue 1 | e54122

Efficient and Reproducible Myogenic Differentiation from Human iPS Cells: Prospects for Modeling Miyoshi Myopathy In Vitro

Akihito Tanaka, Knut Woltjen, Katsuya Miyake, Akitsu Hotta, Makoto Ikeya, Takuya Yamamoto, Tokiko Nishino, Emi Shoji, Atsuko Sehara-Fujisawa, Yasuko Manabe, Nobuharu Fujii, Kazunori Hanaoka, Takumi Era, Satoshi Yamashita, Ken-ichi Isobe, En Kimura, Hidetoshi Sakurai
PLOS ONE. April 2013 | Volume 8 | Issue 4 | e61540

Direct Induction of Chondrogenic Cells from Human Dermal Fibroblast Culture by Defined Factors

Hidetatsu Outani, Minoru Okada, Akihiro Yamashita, Kanako Nakagawa, Hideki Yoshikawa, Noriyuki Tsumaki
PLoS ONE 8(10): e77365

Citrullination regulates pluripotency and histone H1 binding to chromatin

Maria A. Christophorou1, Goncalo Castelo-Branco, Richard P. Halley-Stott, Clara Slade Oliveira,Remco Loos, Aliaksandra Radzisheuskaya, Kerri A. Mowen, Paul Bertone, Jose´ C. R. Silva, Magdalena Zernicka-Goetz,Michael L. Nielsen, John B. Gurdon & Tony Kouzarides
Nature 12942

Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells

Sou Nakamura, Naoya Takayama, Shinji Hirata, Hideya Seo, Hiroshi Endo, Kiyosumi Ochi, Ken-ichi Fujita, Tomo Koike, Ken-ichi Harimoto, Takeaki Dohda, Akira Watanabe, Keisuke Okita, Nobuyasu Takahashi, Akira Sawaguchi, Shinya Yamanaka, Hiromitsu Nakauchi, Satoshi Nishimura, and Koji Eto
Cell Stem Cell 14, 1-14, April 3, 2014

Two miRNA Clusters Reveal Alternative Paths in Late-Stage Reprogramming

Parchem RJ, Ye J, Judson RL, LaRussa MF, Krishnakumar R, Blelloch A, Oldham MC, Blelloch R
Cell Stem Cell Volume 14, Issue 5, 1 May 2014, Pages 617-631

Engineering an in vitro air-blood barrier by 3D bioprinting

Lenke Horváth, Yuki Umehara, Corinne Jud, Fabian Blank, Alke Petri-Fink, & Barbara Rothen-Rutishauser
Scientific Reports. Volume 5, Article number:7974

USA
Nikon Instruments Inc.

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Phone: +1-631-547-8500 : +1-800-52-NIKON (within the U.S.A. only)
Fax: +1-631-547-0306
E-mail: nikoninstruments@nikon.net

Europe
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Phone: +31-20-7099-000
Fax: +31-20-7099-298

ASIA
Nikon Corporation

Shinagawa Intercity Tower C, 2-15-3, Konan, Minato-ku, Tokyo 108-6290 Japan
Phone: +81-3-6433-3705
Fax: +81-3-6433-3785
E-mail: Support.Biostation@nikon.com

Nikon Instruments (Shanghai) Co., Ltd.

26F, Hang Seng Bank Tower, No.1000 Lujiazui Ring Road, Pudong New District, Shanghai 200120, China
Phone: +86-21-6841-2050
Fax: +86-21-6841-2060

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