The ISSCR's annual meeting has become the world's premier stem cell research event. The meeting serves as the largest forum for stem cell and regenerative medicine professionals from around the world. During the exhibition, Nikon will present its advance imaging products and applications. There will also be an Innovation Showcase that will highlight Nikon's Live-cell based drug-screening method for Neuronal degeneration.
VENUE: Boston Convention and Exhibition
Center, Boston, MA, USA
Innovation showcase provided by Nikon
Live cell imaging and morphometric quantification of neuronal degeneration
Professor Lee L. Rubin, PhD
Director of HSCI's Therapeutic Screening Center Professor in Harvard University, USA
- DATE: Thursday, June 15, 2017
- TIME: 8:00 - 8:30 a.m.
- VENUE: Level2, Room 205BC
- E-mail: Support.Biostation@nikon.com
Nikon BioStation CT allows for long term time-lapse imaging of cells using phase contrast and fluorescence.
- Imaging of the direct induction from mouse fibroblasts to Neural stem cells and neurons
Stem Cells. 2012 Jun;30(6) : 1109-19
Professor Hideyuki Okano and Dr. Takeshi Matsui Department of Physiology, Keio University School of Medicine
- Magnification : 4X
- Culture period : 18 days
- Imaging interval : 4 hours
The long term images from the BioStation CT are analyzed using advanced detection and tracking software
Nikon BioStation CT is an advanced, automated cell culture observation instrument specialized for stem cell research.
Ideally suited for live cell imaging, Nikon's BioStation CT maintains a stable environment during microscopic image acquisition.
Whole well scan imaging enables clear detection of iPS cell colonies or differentiated cells, which are difficult to detect because of its low induction efficiency. Advanced image analysis technology facilitates live cell screening, quantitative evaluation and a better understanding of the reprogramming/differentiation process and disease modeling. Imaging and analysis become processed automatically by separate PC with no stress. The left Figure shows the result of automated classification into iPS and non-iPS cell colonies from the phase contrast images. (The entire area of a 100mm dish)
The BioStation CT can incubate up to 30 vessels at one time. Researchers can examine different culture conditions at one time.
User can use various culture vessels (Well-plate, dish, flask)
Temperature, Humidity, CO2 concentration and O2 concentration are controlled by accurate sensor and feedback system.