The whole images of 201B7 cell colonies grown in a 6-well-plate coated with Fibronectin in the presence of drugs in hESF9 medium was measured by an analysis software CL-Quant. This assay can detect each iPS colonies by recognizing the boundary even when confluent.
Magnification : 4X (Phase contrast)
Culture period : 4 days
Imaging interval : 12 hours
Dr. Miho K Furue (Project Leader) and Dr. Masaki Kinehara (2010-2013)
National Institute of Biomedical Innovation (Japan)
PLoS ONE 8(1): e54122. doi:10.1371