Confocal Microscope

A1 HD25/A1R HD25

Highest performance optics for confocal imaging

A selection of high numerical aperture (NA) objectives are available, which provide chromatic aberration correction for UV to near-infrared.

CFI SR HP Plan Apochromat Lambda S 100XC Sil

By using silicone oil that has a refractive index closely matching that of live cells as its immersion liquid, this lens allows high resolution imaging of thick samples and is suitable for long-term time-lapse imaging.

  • Numerical aperture: 1.35
  • Chromatic aberration correction: from visible to UV
  • Nano Crystal Coat applied.

CFI Plan Apochromat 10xC Glyc

This lens has a long working-distance providing ultra-deep imaging with maximum clarity and contrast. The support of multiple refractive indices allows observation with various optical clearing liquids as well as water, oil, and glycerin.

  • Working distance: 5.5 mm
  • Numerical aperture: 0.5
  • Chromatic aberration correction: from visible to infrared.
  • Nano Crystal Coat applied.

CFI Apochromat LWD Lambda S 20XC WI

This highly versatile objective provides superior performance thanks to its high numerical aperture, wide field of view and long working distance.

  • Working distance: 0.95 mm
  • Numerical aperture: 0.95
  • Chromatic aberration correction: from visible to infrared.
  • Nano Crystal Coat applied.

CFI Apochromat Lambda S 40XC WI

Its high NA for water immersion objectives provides brighter and higher-resolution images and makes this lens ideal for confocal live cell imaging.

  • Numerical aperture: 1.25
  • Chromatic aberration correction: from UV through to near IR
  • Nano Crystal Coat applied.

CFI Plan Apochromat IR 60XC WI

This lens has the industry’s highest NA for a 60X water immersion objective lens, maintaining flatness throughout the field of view while showing good correction of chromatic aberration over a wide range of wavelengths.

  • Numerical aperture: 1.27
  • Super image flatness
  • Chromatic aberration correction: from visible to infrared.
  • Nano Crystal Coat applied.

CFI Apochromat TIRF 60XC Oil

This lens has the industry’s highest NA, providing unparalleled resolution and efficient acquisition of fluorescent signals in confocal imaging.

  • Numerical aperture: 1.49

Nano Crystal Coat for superior transmission

Nikon’s exclusive Nano Crystal Coat is an anti-reflective coating consisting of ultra-fine crystalline particles. This forms a coarse structure that enables lower refractive indices, facilitating the passage of light through the lens rather than reflecting it, thus providing superior light transmission.

Objectives for confocal microscopy
CFI Plan Apochromat 10XC Glyc NA 0.50 WD 5.50 Nano Crystal Coat
CFI Plan Apochromat Lambda 10X NA 0.45 WD 4.00 Nano Crystal Coat
CFI Plan Apochromat Lambda 20X NA 0.75 WD 1.00 Nano Crystal Coat
CFI Plan Apochromat Lambda 40XC NA 0.95 WD 0.21 Nano Crystal Coat
CFI Plan Apochromat Lambda 60XC NA 0.95 WD 0.21-0.09 Nano Crystal Coat
CFI Plan Apochromat Lambda 60X Oil NA 1.40 WD 0.13 Nano Crystal Coat
CFI Plan Apochromat Lambda 100X Oil NA 1.45 WD 0.13 Nano Crystal Coat
CFI Plan Apochromat VC 20X NA 0.75 WD 1.00
CFI Plan Apochromat VC 60XC WI NA 1.20 WD 0.31-0.28
CFI Plan Apochromat VC 100X Oil NA 1.40 WD 0.13
CFI Plan Apochromat IR 60XC WI NA 1.27 WD 0.18-0.16 Nano Crystal Coat
CFI SR Plan Apochromat IR 60XC WI NA 1.27 WD 0.18-0.16 Nano Crystal
CFI SR HP Plan Apochromat Lambda S 100XC Sil NA 1.35 WD 0.30 Nano Crystal Coat
CFI Apochromat Lambda S 40XC WI NA 1.25 WD 0.20-0.16 Nano Crystal Coat
CFI Apochromat Lambda S 60X Oil NA 1.40 WD 0.14 Nano Crystal Coat
CFI Apochromat LWD Lambda S 20XC WI NA 0.95 WD 0.95 Nano Crystal Coat
CFI Apochromat LWD Lambda S 40XC WI NA 1.15 WD 0.62-0.58 Nano Crystal Coat
CFI Apochromat TIRF 60XC Oil NA 1.49 WD 0.14-0.08 (23℃)
WD 0.16-0.10 (37℃)
CFI Apochromat TIRF 100XC Oil NA 1.49 WD 0.14-0.08 (23℃)
WD 0.15-0.09 (37℃)
CFI75 Apochromat 25XC W NA 1.10 WD 2.00 Nano Crystal Coat

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