Confocal Microscope

A1+ / A1R+

  1. A1+ Key Features
  2. A1R+ Key Features
  3. GaAsP detector
  4. Imaging software
  5. Objectives
  6. Sample Images
  7. Specifications/Dimensions
A1+ A1R+
Scan head input/output port 2 laser input ports
3 signal output ports for standard, spectral and optional detector*
Laser LU-N3 3-laser unit 405 nm, 488 nm, 561nm lasers are installed; built-in AOTF (Cannot be used with A1-DUS spectral detector)
LU-N4/N4S 4-laser unit 405 nm, 488 nm, 561 nm,640 nm lasers are installed; built-in AOTF (LU-N4 cannot be used with A1-DUS spectral detector)
LU-NV series laser unit Compatible lasers : 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm, 647 nm ; built-in AOTF
Standard fluorescence detector Wavelength 400-750 nm
Detector A1-DU4-2 4 Detector Unit: 4 Multi-Alkali PMTs
A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs
Filter cube 6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75
Diascopic detector (Option) Wavelength 485-650 nm
Detector Multi-Alkali PMT
FOV Square inscribed in a Ø 18 mm circle
Image bit depth 4096 gray intensity levels (12 bit)
Scan head Standard image acquisition Scanner: galvano scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed:
Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction)
Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)**
Zoom: 1-1000x continuously variable
Scan mode: X-Y, X-T, X-Z, XY rotation, Free line, Line-Z
High-speed image acquisition Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis)
Pixel size: max. 1024 x 1024 pixels
Scanning speed: 15 fps (1024 x 1024 pixels), 30 fps (512 x 512 pixels), 60 fps (256 x 256 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed)
Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Scan mode: X-Y, X-T, X-Z
Acquisition method: High-speed image acquisition, Simultaneous photoactivation and image acquisition
Dichroic mirror Low-angle incidence method, Number of positions: 8
Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80
Optional filter: 457/514/561
Pinhole 12-256 µm variable (1st image plane)
Spectral detector (option) A1-DUS spectral detector unit Number of channels: 32
Wavelength detection range: 400 - 750 nm
Spectral image acquisition speed: 4 fps (256 x 256 pixels)
Maximum pixel size: 2048 x 2048 (Spectral mode/Virtual filter mode)
Wavelength resolution: 2.5/6.0/10.0 nm, wavelength range variable in 0.25 nm steps
Compatible with galvano scanner only
A1-DUVB-2 GaAsP detector unit Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (A1-DUVB-OP) with a user-defined dichroic mirror and barrier filter
Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest:320 nm
Maximum pixel size: 4096 x 4096 (CB mode/VB mode)
Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps
Compatible with galvano and resonant scanners
Z step Ti2-E: 0.02 µm, FN1 stepping motor: 0.05 µm
Ni-E: 0.025 µm
Compatible microscopes ECLIPSE Ti2-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type)
Option Motorized XY stage (for Ti2-E/Ni-E), High-speed Z stage (for Ti2-E), High-speed piezo objective-positioning system (for FN1/Ni-E)
Software Display/image generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Application FRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control computer OS Windows 10 Pro 64bit, English version or Japanese version OS Version 1704
Windows 7 Professional, 64bit, SP1 English version or Japanese version Windows Update KB3118401 or later
CPU Intel Xeon E5-2643v4 (3.40GHz, 6 cores, 20MB, 2400MHz) or higher
RAM 16GB, 32GB or 64GB
HDD 1st HP Z Turbo G2 512GB PCIe M.2 SSD
2nd SATA 2TB
Optical Drive Super Multi drive, up to x 16 speed or higher
Graphics NVIDIA Quadro K620/ K2200/ K4200/ M2000/ M4000/ M5000 (PCI Express / two-screen split display supported)
Extension slot Two PCI Express 3.0 (x16) slots (one slot to be used for graphics)
One PCI Express 3.0 (x8 mechanical, x4 electrical) slot
One PCI Express 2.0 (x8 mechanical, x4 electrical) slot
One PCI Express 2.0 (x1) slot
LAN port 10/100/1000 Network/Interface x 2 (for connection to controller, for connection to external LAN)
Monitor 1600 x 1200 or higher resolution, dual monitor configuration recommended
Recommended installation conditions Temperature 23 ± 5 ºC, humidity 70 % (RH) or less (non-condensing)
  • *FCS/FCCS/FLIM is possible in combination with third-party systems
  • **Fast mode is compatible with zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line, CROP, ROI, Spectral imaging, Stimulation and FLIM.

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