Imaging Software

NIS-Elements

  1. Overview
  2. Key Features
  3. Options
  4. NIS-Elements C-ER
  5. NIS-Elements HC
  6. Table of features

NIS-Elements C-ER: Software package for confocal imaging with automatic enhanced resolution imagingConfocal Enhanced Resolution

  • *Includes full functionality of NIS-Elements C

Higher resolution confocal images can be generated with a single click operation. The software assesses the captured image and automatically determines processing parameters to achieve enhanced resolution without sacrificing high imaging operability.
In addition, the resolution of previously captured confocal images can be improved using the pre-processing technology.

Resolution

Image resolution is defined as the smallest distance between 2 points that can be resolved. The theoretical limit of resolution for a conventional optical microscope is approx. 200nm. Confocal microscopes potentially possess the capability of acquiring higher resolution images, but this has not been effectively achieved. With unique image processing technology, image resolution can be increased beyond that of a conventional confocal image (resolution can be improved 1.5 times (XY), 1.7 times (Z)).

Normal confocal image
NIS-Elements C-ER image

Red: Central spindle, Blue: Nuclei
Image courtesy of: Toshinori Hyodo Ph.D., Department of Biochemistry, Aichi Medical University School of Medicine

Normal confocal image
NIS-Elements C-ER image

Stress Fibers (LLC-PK1, Pig Kidney Cells), Green: F-actin, Red: Myosin Heavy Chain
Image courtesy of: Keiju Kamijo Ph.D., Division of Anatomy and Cell Biology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University

Normal confocal image
NIS-Elements C-ER image

Apical surfaces of auditory epithelia of mouse cochleae were stained by Atto-565-phalloidin at postnatal day 2.
Image courtesy of: Dr. Hideru Togashi, Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine.

Zebrafish lens at 5 dpf. Nuclei (green) and actin filaments (red) are visualized with Cytox-green and Rhadamine-conjugated phalloidin, respectively. High magnification images indicate lens fiber cells, which become flat and multi-stacked each other.

Image courtesy of: Drs. Toshiaki Mochizuki and Ichiro Masai, Developmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University

Upgradability

NIS-Elements C-ER is compatible with A1 HD25/A1R HD25 confocal microscopes. An upgrade plan is available for current NIS-Elements C users.

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