Stem cell screening inside the incubator
With conventional cell monitoring procedures, a culture vessel has to be taken out of the incubator for microscope observation, where cells are subjected to stressful environmental changes and vibration. Researchers then have to spend additional time repositioning the vessel to find the same observation points. Nikon's BioStation CT eliminates these problems by providing a stable environment so that the cultures don't suffer while they are being imaged and allowing for a complete trace of the same live cells, including stem cells.
Advanced basic functions
Automatic image capture
Automatic vessel transportation
BioStation CT incorporates a transport unit that provides stable vessel transportation within the heated and humidified incubation area. The high-precision motorized stage in the observation unit allows for automated imaging of the entire area of a well in all culturing formats.
Full-well scan imaging and highly magnified image stitching
High-resolution full-well scans are reconstructed by stitching captured adjacent images. This enables clear detection of an iPS colony, which is difficult to detect because of its low induction efficiency, no matter where it forms in the vessel. The specified position of the vessel can be highly magnified with high resolution. BioStation CT also offers cell registration to allow for repeated visits to the same location. These time-lapse sequences can be created even when a vessel is removed from the BioStation CT for medium exchange.
[Note] Depending on the vessel used, the BioStation CT may not be able to focus on some areas.
Phase contrast and fluorescence images can be captured with the high-sensitivity cooled CMOS camera. These images can be magnified in 2x, 4x, 10x, 20x and 40x. Up to 40 phase contrast images can be captured along the Z axis with the Z-stack function.
Stable culture environment maintenance
Precise temperature control
Humidity control with air-flow type active aerosol spray humidifier
Environment data recording
Smooth vessel transportation
Reduced contamination risk
The incubator interior can be sterilized using hydrogen peroxide gas. (This is optional, and a 200 V power source is necessary.)
Different GUI for each vessel type
1. Vessel installation
Culture vessel installation into the storage rackVessels are placed in the incubator through a small door on the front glass panel, minimizing the effect on the environment within the incubator.
2. Imaging parameter setting
Easy touchscreen operationTime-lapse imaging configurations such as magnification, imaging point, fluorescence channel and stage motion speed can be set.
Time-lapse imaging scheduleThe imaging interval and total period can be set. The shortest time-lapse imaging interval is one minute.
4. Captured image view
Culture history data managementThe time-course change of a specimen can be observed easily in sequentially displayed captured images.
5. Medium exchange
High-precision repeatabilityAccurate tracing of same cells, even after medium exchange, is possible using a dedicated tray holder, as BioStation CT records culture history, such as medium exchange and subculture, as well as X-Y positions for each vessel.
6. Data report
Reliable data management and documentation supportObtained data is duplicated and protected using uninterruptible power supply. Observation information such as temperature, humidity and imaging date can be written and displayed on the captured image to simplify presentation document preparation.