Nikon Super Resolution Microscope N-STORM 4.0 optimized for dynamic nanoscale imaging

March 19, 2015

Nikon Corporation (Kazuo Ushida, President, Minato-ku, Tokyo) is pleased to announce the release of the Super Resolution Microscope N-STORM 4.0 at Focus On Microscopy (March 29- April 1, Gottingen, Germany). N-STORM 4.0 extends the capability of dynamic nanoscale imaging of conventional N-STORM to open up a new world of science.

Product information

Product Name Super Resolution Microscope N-STORM 4.0
Available from the end of May 2015

Product Concept

Nikon has established a leading position in the field of super resolution microscopy since Super Resolution Microscope N-STORM was released in 2010. N-STORM provides the capability to obtain images with approximately 10 times higher resolution than conventional optical microscopes.
N-STORM 4.0 has been developed in response to the increasing importance of super resolution imaging in life science spotlighted by 2014 Novel Prize in Chemistry awarded to three researchers in this field. N-STORM 4.0 now has the capability of acquiring super resolution images of live cell phenomenon, which has never been achieved with conventional N-STORM.


1. Dynamic Super Resolution Imaging at the nanoscale level

N-STORM 4.0 improves image acquisition speed from minutes to seconds*2 for 1 shot with a new sCMOS camera*3, which is capable of approximately 10 times faster image acquisition than before. Thanks to this improvement, it is now possible to acquire images of living samples dynamically at 20nm resolution.

2. Improvement of Image Quality

N-STORM utilizes positional information from single molecules to construct the images. For that reason, it is important to enhance the density of molecules per unit area to improve the image quality. Thanks to the improved laser excitation method and the increased image acquisition frame, N-STORM 4.0 provides much clearer images with high molecule counts. Also, Wide-view mode is achieved at 80μm x 80μm which is 4 times wider imaging area than conventional.

Comparison N-STORM 4.0 and Conventional N-STORM

Fluorescence observation by N-STORM 4.0
Fluorescence observation by conventional N-STORM

Sample: Tubulin*4 of BSC-1*5 cell labeled with Alexa Fluor® 647, acquisition time: 20 seconds

Alexa Fluor is a registered trademark of Thermo Fisher Scientific, Inc.

This information is current as of the date of publication. It is subject to change without notice.